mouse anti h2ax Search Results


anti h2ax ps139 apc antibody  (Miltenyi Biotec)
95
Miltenyi Biotec anti h2ax ps139 apc antibody
Anti H2ax Ps139 Apc Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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histone h2ax [p ser139] antibody (3f2)  (Bio-Techne corporation)
94
Bio-Techne corporation histone h2ax [p ser139] antibody (3f2)
Histone H2ax [P Ser139] Antibody (3f2), supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti h2ax ps139 fitc  (Miltenyi Biotec)
95
Miltenyi Biotec anti h2ax ps139 fitc
Anti H2ax Ps139 Fitc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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γh2ax  (Miltenyi Biotec)
95
Miltenyi Biotec γh2ax
EA treatment alters mitochondrial dynamics, leading to the accumulation of intracellular ROS and DNA damage. Western blot analysis of the protein levels of Mfn2, Drp1, total-ERK, and p-ERK in the (A) A2780 monolayer, (B) A2780 spheroids, and (C) SKOV3 spheroids cells; A549-CD133 + (D,E) and SKOV3- spheroids (F,G) were treated with different concentrations of EA (0, 5, 10, 25 μM) for 24 h. Intracellular ROS accumulation was determined using flow cytometry. Representative histograms and corresponding bar diagrams showing ROS level in CSLCs; A549-CD133 + (H) and SKOV3- spheroid (I) cells were treated with different doses of EA for 48 h. <t>γH2AX</t> accumulation was detected through flow cytometry to evaluate the extent of DNA damage induced by EA. Representative contour plots obtained from flow cytometry analysis showing the percentage of γH2AX positive cells. Data was reanalyzed using FlowJo software. In all graphs, the results are represented as mean ± SD of triplicate experiments. ** P < 0.01, and *** P < 0.001.
γh2ax, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/γh2ax/product/Miltenyi Biotec
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anti h2ax ps139 rea502  (Miltenyi Biotec)
95
Miltenyi Biotec anti h2ax ps139 rea502
EA treatment alters mitochondrial dynamics, leading to the accumulation of intracellular ROS and DNA damage. Western blot analysis of the protein levels of Mfn2, Drp1, total-ERK, and p-ERK in the (A) A2780 monolayer, (B) A2780 spheroids, and (C) SKOV3 spheroids cells; A549-CD133 + (D,E) and SKOV3- spheroids (F,G) were treated with different concentrations of EA (0, 5, 10, 25 μM) for 24 h. Intracellular ROS accumulation was determined using flow cytometry. Representative histograms and corresponding bar diagrams showing ROS level in CSLCs; A549-CD133 + (H) and SKOV3- spheroid (I) cells were treated with different doses of EA for 48 h. <t>γH2AX</t> accumulation was detected through flow cytometry to evaluate the extent of DNA damage induced by EA. Representative contour plots obtained from flow cytometry analysis showing the percentage of γH2AX positive cells. Data was reanalyzed using FlowJo software. In all graphs, the results are represented as mean ± SD of triplicate experiments. ** P < 0.01, and *** P < 0.001.
Anti H2ax Ps139 Rea502, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti h2ax ps139 rea502/product/Miltenyi Biotec
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biotinylated mouse anti-phospho-h2ax (ser139) mab  (Merck & Co)
90
Merck & Co biotinylated mouse anti-phospho-h2ax (ser139) mab
EA treatment alters mitochondrial dynamics, leading to the accumulation of intracellular ROS and DNA damage. Western blot analysis of the protein levels of Mfn2, Drp1, total-ERK, and p-ERK in the (A) A2780 monolayer, (B) A2780 spheroids, and (C) SKOV3 spheroids cells; A549-CD133 + (D,E) and SKOV3- spheroids (F,G) were treated with different concentrations of EA (0, 5, 10, 25 μM) for 24 h. Intracellular ROS accumulation was determined using flow cytometry. Representative histograms and corresponding bar diagrams showing ROS level in CSLCs; A549-CD133 + (H) and SKOV3- spheroid (I) cells were treated with different doses of EA for 48 h. <t>γH2AX</t> accumulation was detected through flow cytometry to evaluate the extent of DNA damage induced by EA. Representative contour plots obtained from flow cytometry analysis showing the percentage of γH2AX positive cells. Data was reanalyzed using FlowJo software. In all graphs, the results are represented as mean ± SD of triplicate experiments. ** P < 0.01, and *** P < 0.001.
Biotinylated Mouse Anti Phospho H2ax (Ser139) Mab, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti- γ-h2ax mouse monoclonal antibody  (BioAcademia)
90
BioAcademia anti- γ-h2ax mouse monoclonal antibody
EA treatment alters mitochondrial dynamics, leading to the accumulation of intracellular ROS and DNA damage. Western blot analysis of the protein levels of Mfn2, Drp1, total-ERK, and p-ERK in the (A) A2780 monolayer, (B) A2780 spheroids, and (C) SKOV3 spheroids cells; A549-CD133 + (D,E) and SKOV3- spheroids (F,G) were treated with different concentrations of EA (0, 5, 10, 25 μM) for 24 h. Intracellular ROS accumulation was determined using flow cytometry. Representative histograms and corresponding bar diagrams showing ROS level in CSLCs; A549-CD133 + (H) and SKOV3- spheroid (I) cells were treated with different doses of EA for 48 h. <t>γH2AX</t> accumulation was detected through flow cytometry to evaluate the extent of DNA damage induced by EA. Representative contour plots obtained from flow cytometry analysis showing the percentage of γH2AX positive cells. Data was reanalyzed using FlowJo software. In all graphs, the results are represented as mean ± SD of triplicate experiments. ** P < 0.01, and *** P < 0.001.
Anti γ H2ax Mouse Monoclonal Antibody, supplied by BioAcademia, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-γ-h2ax primary antibody mouse anti-γh2ax (ser139)  (Stressgen Biotechnologies)
90
Stressgen Biotechnologies anti-γ-h2ax primary antibody mouse anti-γh2ax (ser139)
EA treatment alters mitochondrial dynamics, leading to the accumulation of intracellular ROS and DNA damage. Western blot analysis of the protein levels of Mfn2, Drp1, total-ERK, and p-ERK in the (A) A2780 monolayer, (B) A2780 spheroids, and (C) SKOV3 spheroids cells; A549-CD133 + (D,E) and SKOV3- spheroids (F,G) were treated with different concentrations of EA (0, 5, 10, 25 μM) for 24 h. Intracellular ROS accumulation was determined using flow cytometry. Representative histograms and corresponding bar diagrams showing ROS level in CSLCs; A549-CD133 + (H) and SKOV3- spheroid (I) cells were treated with different doses of EA for 48 h. <t>γH2AX</t> accumulation was detected through flow cytometry to evaluate the extent of DNA damage induced by EA. Representative contour plots obtained from flow cytometry analysis showing the percentage of γH2AX positive cells. Data was reanalyzed using FlowJo software. In all graphs, the results are represented as mean ± SD of triplicate experiments. ** P < 0.01, and *** P < 0.001.
Anti γ H2ax Primary Antibody Mouse Anti γh2ax (Ser139), supplied by Stressgen Biotechnologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-mouse–hrp antibody for the detection of h2ax histone  (Amersham Life Sciences Inc)
90
Amersham Life Sciences Inc anti-mouse–hrp antibody for the detection of h2ax histone
EA treatment alters mitochondrial dynamics, leading to the accumulation of intracellular ROS and DNA damage. Western blot analysis of the protein levels of Mfn2, Drp1, total-ERK, and p-ERK in the (A) A2780 monolayer, (B) A2780 spheroids, and (C) SKOV3 spheroids cells; A549-CD133 + (D,E) and SKOV3- spheroids (F,G) were treated with different concentrations of EA (0, 5, 10, 25 μM) for 24 h. Intracellular ROS accumulation was determined using flow cytometry. Representative histograms and corresponding bar diagrams showing ROS level in CSLCs; A549-CD133 + (H) and SKOV3- spheroid (I) cells were treated with different doses of EA for 48 h. <t>γH2AX</t> accumulation was detected through flow cytometry to evaluate the extent of DNA damage induced by EA. Representative contour plots obtained from flow cytometry analysis showing the percentage of γH2AX positive cells. Data was reanalyzed using FlowJo software. In all graphs, the results are represented as mean ± SD of triplicate experiments. ** P < 0.01, and *** P < 0.001.
Anti Mouse–Hrp Antibody For The Detection Of H2ax Histone, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alexa fluor 555 anti-mouse h2ax antibody  (Cedarlane)
90
Cedarlane alexa fluor 555 anti-mouse h2ax antibody
EA treatment alters mitochondrial dynamics, leading to the accumulation of intracellular ROS and DNA damage. Western blot analysis of the protein levels of Mfn2, Drp1, total-ERK, and p-ERK in the (A) A2780 monolayer, (B) A2780 spheroids, and (C) SKOV3 spheroids cells; A549-CD133 + (D,E) and SKOV3- spheroids (F,G) were treated with different concentrations of EA (0, 5, 10, 25 μM) for 24 h. Intracellular ROS accumulation was determined using flow cytometry. Representative histograms and corresponding bar diagrams showing ROS level in CSLCs; A549-CD133 + (H) and SKOV3- spheroid (I) cells were treated with different doses of EA for 48 h. <t>γH2AX</t> accumulation was detected through flow cytometry to evaluate the extent of DNA damage induced by EA. Representative contour plots obtained from flow cytometry analysis showing the percentage of γH2AX positive cells. Data was reanalyzed using FlowJo software. In all graphs, the results are represented as mean ± SD of triplicate experiments. ** P < 0.01, and *** P < 0.001.
Alexa Fluor 555 Anti Mouse H2ax Antibody, supplied by Cedarlane, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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histone h2ax antibody  (Bio-Techne corporation)
90
Bio-Techne corporation histone h2ax antibody
EA treatment alters mitochondrial dynamics, leading to the accumulation of intracellular ROS and DNA damage. Western blot analysis of the protein levels of Mfn2, Drp1, total-ERK, and p-ERK in the (A) A2780 monolayer, (B) A2780 spheroids, and (C) SKOV3 spheroids cells; A549-CD133 + (D,E) and SKOV3- spheroids (F,G) were treated with different concentrations of EA (0, 5, 10, 25 μM) for 24 h. Intracellular ROS accumulation was determined using flow cytometry. Representative histograms and corresponding bar diagrams showing ROS level in CSLCs; A549-CD133 + (H) and SKOV3- spheroid (I) cells were treated with different doses of EA for 48 h. <t>γH2AX</t> accumulation was detected through flow cytometry to evaluate the extent of DNA damage induced by EA. Representative contour plots obtained from flow cytometry analysis showing the percentage of γH2AX positive cells. Data was reanalyzed using FlowJo software. In all graphs, the results are represented as mean ± SD of triplicate experiments. ** P < 0.01, and *** P < 0.001.
Histone H2ax Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human/mouse/rat histone h2ax antibody  (Bio-Techne corporation)
93
Bio-Techne corporation human/mouse/rat histone h2ax antibody
EA treatment alters mitochondrial dynamics, leading to the accumulation of intracellular ROS and DNA damage. Western blot analysis of the protein levels of Mfn2, Drp1, total-ERK, and p-ERK in the (A) A2780 monolayer, (B) A2780 spheroids, and (C) SKOV3 spheroids cells; A549-CD133 + (D,E) and SKOV3- spheroids (F,G) were treated with different concentrations of EA (0, 5, 10, 25 μM) for 24 h. Intracellular ROS accumulation was determined using flow cytometry. Representative histograms and corresponding bar diagrams showing ROS level in CSLCs; A549-CD133 + (H) and SKOV3- spheroid (I) cells were treated with different doses of EA for 48 h. <t>γH2AX</t> accumulation was detected through flow cytometry to evaluate the extent of DNA damage induced by EA. Representative contour plots obtained from flow cytometry analysis showing the percentage of γH2AX positive cells. Data was reanalyzed using FlowJo software. In all graphs, the results are represented as mean ± SD of triplicate experiments. ** P < 0.01, and *** P < 0.001.
Human/Mouse/Rat Histone H2ax Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


EA treatment alters mitochondrial dynamics, leading to the accumulation of intracellular ROS and DNA damage. Western blot analysis of the protein levels of Mfn2, Drp1, total-ERK, and p-ERK in the (A) A2780 monolayer, (B) A2780 spheroids, and (C) SKOV3 spheroids cells; A549-CD133 + (D,E) and SKOV3- spheroids (F,G) were treated with different concentrations of EA (0, 5, 10, 25 μM) for 24 h. Intracellular ROS accumulation was determined using flow cytometry. Representative histograms and corresponding bar diagrams showing ROS level in CSLCs; A549-CD133 + (H) and SKOV3- spheroid (I) cells were treated with different doses of EA for 48 h. γH2AX accumulation was detected through flow cytometry to evaluate the extent of DNA damage induced by EA. Representative contour plots obtained from flow cytometry analysis showing the percentage of γH2AX positive cells. Data was reanalyzed using FlowJo software. In all graphs, the results are represented as mean ± SD of triplicate experiments. ** P < 0.01, and *** P < 0.001.

Journal: ACS Omega

Article Title: Ellagic Acid Induces DNA Damage and Apoptosis in Cancer Stem-like Cells and Overcomes Cisplatin Resistance

doi: 10.1021/acsomega.3c08819

Figure Lengend Snippet: EA treatment alters mitochondrial dynamics, leading to the accumulation of intracellular ROS and DNA damage. Western blot analysis of the protein levels of Mfn2, Drp1, total-ERK, and p-ERK in the (A) A2780 monolayer, (B) A2780 spheroids, and (C) SKOV3 spheroids cells; A549-CD133 + (D,E) and SKOV3- spheroids (F,G) were treated with different concentrations of EA (0, 5, 10, 25 μM) for 24 h. Intracellular ROS accumulation was determined using flow cytometry. Representative histograms and corresponding bar diagrams showing ROS level in CSLCs; A549-CD133 + (H) and SKOV3- spheroid (I) cells were treated with different doses of EA for 48 h. γH2AX accumulation was detected through flow cytometry to evaluate the extent of DNA damage induced by EA. Representative contour plots obtained from flow cytometry analysis showing the percentage of γH2AX positive cells. Data was reanalyzed using FlowJo software. In all graphs, the results are represented as mean ± SD of triplicate experiments. ** P < 0.01, and *** P < 0.001.

Article Snippet: Washing was done twice with 1× PBS and staining was performed using the γH2AX (Miltenyi Biotec cat. no. 130-130-829) antibody.

Techniques: Western Blot, Flow Cytometry, Software

Combinatorial treatment of EA and cisplatin impairs DNA damage repair in lung and ovarian CSLCs. The DNA repair kinetics study depicts the percent DNA damage accumulation following DMSO, EA, cisplatin, and EA + cisplatin treatment for 12 h followed by 3 h damage recovery in (A) A549-CD133 + and (B) SKOV3 spheroid cells. (C) The immunofluorescence staining shows the increasing accumulation of DNA damage in DMSO, EA, cisplatin, and EA + cisplatin-treated cells. The corresponding graph represents the mean fluorescence intensity of accumulated γH2AX. N=3, Bar, SD; * P < 0.05, ** P < 0.01, and *** P < 0.001.

Journal: ACS Omega

Article Title: Ellagic Acid Induces DNA Damage and Apoptosis in Cancer Stem-like Cells and Overcomes Cisplatin Resistance

doi: 10.1021/acsomega.3c08819

Figure Lengend Snippet: Combinatorial treatment of EA and cisplatin impairs DNA damage repair in lung and ovarian CSLCs. The DNA repair kinetics study depicts the percent DNA damage accumulation following DMSO, EA, cisplatin, and EA + cisplatin treatment for 12 h followed by 3 h damage recovery in (A) A549-CD133 + and (B) SKOV3 spheroid cells. (C) The immunofluorescence staining shows the increasing accumulation of DNA damage in DMSO, EA, cisplatin, and EA + cisplatin-treated cells. The corresponding graph represents the mean fluorescence intensity of accumulated γH2AX. N=3, Bar, SD; * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: Washing was done twice with 1× PBS and staining was performed using the γH2AX (Miltenyi Biotec cat. no. 130-130-829) antibody.

Techniques: Immunofluorescence, Staining, Fluorescence